The Knights Hospitalier were one of the several knightly orders founded during the Crusades, and were tasked to provide care for the sick and wounded in the Holy Land.
By 1191, the Knights were led by Garnier de Naplouse, who was secretly a [[Templars|Templar]]. He experimented on his patients to try and replicate the effects of the [[Apple of Eden 2|Apple of Eden]]. He achieved a limited amount of success, though he was assassinated by [[Altaïr Ibn-La'Ahad]], which ended his experiments.
By the Renaissance, the Hospitalier base had moved to [[Rhodes]], and the knightly order were responsible for killing the [[Master Assassin]] [[Castor]]. In response, [[Ezio Auditore da Firenze]] sent a team of [[Turkish Assassins|Ottoman Assassins]] to burn the Hospitalier compound to the ground.
The Knights Hospitalier were the only faction of Crusaders without high-ranking soldiers, aside from informant targets.
In Assassin's Creed, the armor and uniform of Knights Hospitalier guards was strikingly similar to that of the high-ranking Templar crusaders in the Holy Land, with the primary difference being the black and white coloring of the Hospitaliers, as opposed to the red and white of the Templar crusaders.