This segment begins with the War of the Spanish Succession, the background to the Golden Age of Piracy in which privateering first came into formation in the Caribbean. The Encyclopedia introduces the Welsh privateer turned pirate Edward Kenway and his conflict with the rogue Assassin Duncan Walpole. The events of Edward's life are uncovered in detail beginning with his early exploits, such as his failed infiltration of the Templar Order, his imprisonment aboard the Spanish treasure fleet and subsequent escape aboard a vessel he renamed the Jackdaw.
This section also explores Edward's interactions with both the Assassins and Templars and his general reluctance to support either ideology. The Encyclopedia highlights Edward's focus on furthering his own wealth and stature instead, which inevitably brings him into conflict with both factions as they attempt to uncover the location of the fabled Observatory, a valuable site which Edward, believing will grant him excessive wealth, is already searching for. This segment also covers Edward's relationships with the other major pirates of the era such as Edward Thatch, James Kidd, Anne Bonny, Benjamin Hornigold, Stede Bonnet, Calico Jack, Charles Vane and Bartholomew Roberts, as well as the rise and fall of the pirate republic established at Nassau in the Bahamas.
This section begins with the arrival of Templar Grand Master Haytham Kenway in America prior to the commencement of the Seven Years' War, and details his search for the precursor site with other notable Templars of the region including Charles Lee, Benjamin Church, William Johnson, John Pitcairn and Thomas Hickey. Moving on, the Encyclopedia outlines the notable events participated in by Haytham's son, the Kanien'kehá:ka Assassin Ratonhnhaké:ton in the lead up to the Revolution, such as the Boston Massacre and the Boston Tea Party.
The Encyclopedia then follows his participation in some of the major events of the War for Independence, including the Battles of Lexington, Concord and Bunker Hill, the Declaration of Independence and the Battle of Monmouth. It details his assassinations of the key Templar figures under Haytham's command and subsequent attempts to support the colonists' desire for independence. In doing so the Encyclopedia also explores Ratonhnhaké:ton's relationship with his father Haytham and his gradual disillusionment with the patriot cause after discovering their distain for the native population. This section is concluded with the end of the war and the relocation of Native Americans after the colonists gained their independence from Britain.
This section introduces the origins of the slave society present in New Orleans during the 18th century, before covering the transition from French to Spanish rule in Louisiana following the end of the Seven Years' War and the subsequent rebellion of 1768. This section also introduces the Assassin Aveline de Grandpré and covers her activities in New Orleans as well as her expeditions to Chichen Itza in search of the Prophecy Disk. Her search for the Company Man and meeting with Connor in New York are also described along with her return to New Orleans and confrontation with her stepmother.
This section details the events that took place during the time of Nikolai Orelov, and includes the conflict between the Assassins and Tsars of Russia, namely Alexander III and his son Nicholas II. This section also includes the destruction of the Staff of Eden in the Tunguska explosion and Nikolai's discovery of a shard from the artifact being held in the possession of Grigori Rasputin.
现代历史段落讲述了刺客与圣殿骑士在现代的冲突，故事开始于2000年的大清洗。随后延伸到戴斯蒙德·迈尔斯的故事，始于他被阿布斯泰戈绑架以及随后同露西·斯蒂尔曼逃走。Accompanying this, it describes the discovery of Clay Kaczmarek's Cryptic Messages and later his Glyphs hidden inside the Animus, as well as the increasing intensity of the Bleeding Effect experienced by Desmond as part of his Animus sessions. This section also covers the relocation of the Assassins to the Sanctuary beneath Monteriggioni, the discovery of the Apple of Eden beneath the Colosseum and the death of Lucy at the hands of Desmond, seemingly under the control of Juno. Following this, the Encyclopedia details Desmond's coma and subsequent recovery inside the Black Room with the help of Clay before describing his arrival at the Grand Temple and his travels to Manhattan, Italy and Brazil in search of the temple's power sources. Desmond's story is concluded with his sacrifice inside the temple vault and the prevent of the second catastrophe.
This section on present day also includes the involvement of MysoreTech employee Jot Soora and his fiancée Monima Das in the conflict between the modern day Assassins and Templars in search of the Koh-i-Noor diamond. This section also details the deaths of the Assassins Siobhan Dhami and Dinesh at the hands of Templar commandos, as well as that of Monima, who perished in the waters of Mumbai Bay during a high speed chase with the Templars. This section also includes a description of the Brahman V.R. project and the device's use as a portable Animus. The final events of recent history covered in this section are those that took place in Abstergo Entertainment during the Sample 17 Project, including the infiltration of the complex by Assassins Rebecca Crane and Shaun Hastings, the failed attempt by the Sage John Standish to kill the Abstergo Entertainment research analyst and the subsequent death of John at the hands of Abstergo security guards.
The Characters section contains profiles on all the characters features in the series, beginning with Desmond Miles and the allies and enemies he encountered. After this, it details the ancestors of Desmond, including Aquilus, Ezio Auditore da Firenze and Altaïr Ibn-La'Ahad.
The profile of Aquilus describes the confrontations between him and the Templars, both in their struggle for the Shroud of Eden and the Ankh.
Altaïr's profile is expansive and details his entire life, finishing with his death inside his library in 1257. After his profile, it gives information on all of his assassination targets, allies and other enemies he combated within his time.
Ezio's profile is quite large compared to Altaïr's. It once again details his life and the allies and enemies he encountered. It adds on, however, with explanations of the Venetian and Florentine conspirators and the confrontation in Constantinople.
Ratonhnhaké:ton's profile details his life throughout Assassin's Creed III. Similar to Ezio's profile, it details his life, friends, the allies and enemies he encountered during the events in Assassin's Creed III, the latter such as the American Revolutionary War.
Aveline de Grandpré's profile is much the same as Connor's in that it details her life throughout Assassin's Creed III: Liberation, the friends & allies and enemies she encountered along the way.
Daniel Cross' section outlines how the Templars experimented on him during his youth and his infiltration of the Assassins, whilst including profiles on the people he encountered, including Hannah Mueller, Paul Bellamy and the Mentor.
- On the spine of the Encyclopedia, there was a hidden message written in Unicode. Once translated, and after removing the title in red, the message read "Three lives toiled. Three lives lost. Three messages delivered: the greeting, the warning, the revelation."
- At E3 2012, new editions of the Encyclopedia featured a quote from the Bible written in hexadecimal code instead: "We saw the Nephilim there; We seemed like grasshoppers in our own eyes; And we looked the same to them." (Numbers 13:33). This quote is also spoken by William Miles to Desmond in Assassin's Creed III, after the latter complained about Juno only ever leaving cryptic messages.
- On multiple pages, a molecule was drawn with a benzene ring and a hydrogen atom with two bonds. However, since hydrogen has only one electron to react with, it cannot form a double bond.
- The encyclopedia mainly uses concept art as pictures, as opposed to CGI renders.