Wolves were a common threat in Scandinavia, and they made their way into Norse mythology as well, in the form of Fenrir, a mighty wolf, whose ability to hunt down its prey was emulated by Viking children in the 9th century CE.
5th century BCE
During the Peloponnesian War, wolves inhabited various regions of Greece. Members of the Daughters of Artemis used tamed wolves to protect their territories and to accompany them on hunting trips. The Spartan misthios Kassandra also learned to tame wolves. She also hunted them, for their fur was valued at 40 drachmae apiece, and their fangs at 7 drachmae apiece.
The leader of the Daughters at the time, Daphnae, tasked Kassandra with hunting down the legendary Lykaon Wolf, amongst other legendary animals, and obtaining its pelt. One of the Two Kings of Sparta, Archidamos also tasked Kassandra to slay the beast, demanding its head as proof of the deed.
One of the members of the Cult of Kosmos, Zoisme of the Worshippers of the Bloodline, preferred living among wild animals, and was said to be accompanied by wolves, feeding her victims to them. This practice led to her eviction from Phokis, and she moved to the den of a white bear in Malis. Eventually Kassandra hunted her down and ended her reign of terror. 
The Templars also saw the potency of the wolf symbolism to the Assassins' preoccupation with eagles and other birds: one such example consisted of Baltasar de Silva and Fiora Cavazza dubbing their Assassin-styled protégé "Il Lupo" ('The Wolf'). Akin to this, the Templar Shay Cormac's ship, the Morrigan, featured lupine design on her sails, figurehead and wheel.
During the 18th century, the Assassin Ratonhnhaké:ton became adept at hunting wolves, trading their pelts, meat and teeth for money. When he and Robert Faulkner arrived at Oak Island to search for William Kidd's treasure in 1777, they were attacked by a pack of wolves who were situated near the sinkhole it was buried under.
In an alternate reality, Ratonhnhaké:ton drank the Tea of the Red Willow and became one with the Spirit of the Wolf. In return, he gained the ability to turn invisible, and the power to summon wolves to attack his enemies.
Crawford Starrick, the Grand Master of the British Rite of the Templar Order, also used a stylized likeness of a wolf's head as part of his business symbol, most notably as the logo of the Starrick Telegraph Company.
Behind the scenes
In Assassin's Creed III, Edward Braddock stated "wolves often travel in packs" when he met Haytham Kenway and his fellow Templars in Boston. At Davenport Homestead, wolves could be seen attacking cattle on Warren and Prudence's farm during winter.
- Assassin's Creed: Brotherhood (mentioned only)
- Assassin's Creed III (first appearance)
- Assassin's Creed: Project Legacy (mentioned only)
- Assassin's Creed: Rogue (mentioned only)
- Assassin's Creed: Odyssey
- Assassin's Creed: Valhalla
- Assassin's Creed: Odyssey – The Lykaon Wolf
- Assassin's Creed: Brotherhood
- Assassin's Creed: Valhalla – Hide and Hunt
- Assassin's Creed: Rogue
- Assassin's Creed: Odyssey
- Assassin's Creed: Odyssey – A Legendary Hunt
- Assassin's Creed: Odyssey – Cult of Kosmos clues: The Worshippers of the Bloodline: Zoisme
- Assassin's Creed: Odyssey – The Worshippers of the Bloodline
- Assassin's Creed: Odyssey – Legacy of the First Blade: Hunted
- Assassin's Creed: Odyssey – Repairing the Lyre
- Assassin's Creed: Project Legacy
- Assassin's Creed: Rebellion
- Assassin's Creed III
- Assassin's Creed III – Oak Island (memory)
- Assassin's Creed III – The Tyranny of King Washington: The Infamy
- Assassin's Creed: Syndicate