During the reign of pharaoh Ptolemy XII Auletes, an earthquake struck the city, damaging and altering the structure of the tomb. As a result, the entrance was blocked, preventing access to the inner tomb.
Years later, in 47 BCE, Cleopatra enlisted the aid of Aya and her husband Bayek to gain access to the tomb so that she could use it to impress her lover Julius Caesar and gain his support. The two Medjay were able to locate another entrance to the tomb and remove the blockade, allowing Caesar and Cleopatra access into the inner tomb.
Shortly after the Battle of the Nile, when Cleopatra was proclaimed the sole ruler of Egypt, Flavius Metellus, revealed to be a member of the Order of the Ancients, broke into the tomb alongside Lucius Septimius and stole the Staff. In the process, they also stole the Apple of Eden from Apollodorus, who discovered their true purpose in the tomb. Dispatching soldiers to deal with Apollodorus, the two members of the Order left the tomb and made their way to the vault in Siwa. Both Aya and Bayek arrived too late to stop them and save Apollodorus, who succumbed to his injuries sustained during the fight.
Located in the center of the room, Alexander the Great's sarcophagus had several inscriptions around the outside;
- ΑΜΩΝ ΕΝ ΑΓΡΩΙ ΔΕΝΔΡΩΝ ΙΣΤΗΣΙΝ. ΤΟ ΕΝ ΣΙΒΑΙ ΙΕΡΟΝ ΚΡΑΤΙΣΤΗΝ ΕΞΟΥΣIΑΝ ΚΑΤΕΧΕΙ (Amun stands in a field of trees. The Sanctuary in Siwa holds the highest authority)
- ΑΝΘΡΩΠΟΣ ΠΡΟΣ ΒΑΣΙΛΕΑΝ ΚΑΙ ΒΑΣΙΛΕΥΣ ΠΡΟΣ ΘΕΟΝ ΔΥΝΑΜIΝ ΘΕΩΝ ΕΝ ΧΕΡΣIΝ ΑΝΘΡΩΠΟΥ ΣΤΡΕΦΕΙ (From man to king and from king to god, (he) handles god-like might with (his) mortal hands)
- ΟΦΘΑΛΜΟΣ ΕΗΣ ΚΩΠΗΣ ΤΥΓΧΑΝΕΙ ΕΣΦΡΑΓΙΣΜΕΝΗ ΣΦΑΙΡΑ ΕΠΙ ΤΗΣ ΡΑΒΔΟΥ ΤΕΘΕΙΣΑ (The eye of its handle has a marked sphere set on the staff)
- ΤΑΞΙΣ ΑΠΑNΤΑ ΑNΑΓKΑZΕI ΚΑΙ ΤIΣ ΠΑNΤOΣ ΔYNΑΤΑI KPΑΤΕIN ΕI KΑΤΩPHΞ ΑNΕΩΙΚΤΑΙ (roughly translates to: the order compels everything and one is surely able to have sway if [...] has been disclosed)