In 361 BCE, Tivoli, then known as Tibur, was one of the village that allied itself with the Gauls, remaning peaceful for 23 years. The village was then absorbed into Rome after the Gauls were defeated. In 90 BCE, the city received official Roman citizenship, becoming a popular resort destination for the Roman elite due to its beauty and superb water.
By the Renaissance, the city became known for its open-air travertine mines. Stones were foten excavated to build the palaces and courtyards of Rome. Rumours soon emerge of a treasure buried in one of the mines, although treasure hunters were unable to locate it.
During the early 16th century, the Assassin Ezio Auditore da Firenze visited the aqueducts of Tivoli, in order to assassinate a Papal guard who had been forcing civilians to repair the aqueducts, and to retrieve a hidden treasure from the Templars.