Taimhotep was born as the eldest daughter of a noble family in Memphis and was highly religious, fascinated by the pomp of the temple ritual. She played an important ceremonial role as the High Priest's wife and also served as a counterweight to the pressures of her husband.
Around 48 BCE, Memphis seemed to fall out of favor with the gods, for numerous maladies plagued the city. An unpleasant smell permeated the place, crocodiles came in greater numbers than normal, and they were more eager to attack the citizens and wildlife alike. 
The sacred Apis bull himself fell sick, and Taimhotep suffered multiple miscarriages through the year. After another pregnancy had come far enough to show, Taimhotep argued with her husband at the Temple of Ptah; this encounter was witnessed by the Medjay Bayek of Siwa, who had come to Memphis in order to hunt down a member of the Order of the Ancients, the one known as the Lizard.
Taimhotep stormed off, seeking out The Seer; at Pasherenptah's request, Bayek followed her. At the Seer's house, Bayek participated in a ritual in order to save the child Taimhotep carried, while the Seer chanted with Taimhotep's maidservant, Mara.
Afterwards, Taimhotep retreated to a garden, and Bayek followed the sound of her singing and weeping. He attempted to comfort her, but she was inconsolable. Her maidservant, Mara, took Bayek aside and asked if he would help them perform a protective ritual. He agreed, and Mara asked him to fetch some heron feathers and a fertility talisman; after retrieving them, Bayek met Mara and Taimhotep on the road to the pyramids.
They approached the Pyramid of Djoser, and once there, Taimhotep and Mara prepared for the ritual. Bayek stood guard, alone, for Taimhotep wanted to keep the whole thing as much a secret as possible.
- Historically Taimhotep had four children, three daughters born early in her marriage and a son born in 46 BCE after praying to Imhotep, the deified architect of Djoser's Pyramid. She was also the step-mother of a daughter from a previous relationship of Pasherenptah.