Painted circa 1480, Leonardo's Saint Jerome in the Wilderness was eventually purchased by Ezio Auditore da Firenze and placed in the gallery at the Villa Auditore. The painting was later lost during the Papal attack on Monteriggioni, led by the Templar Cesare Borgia.
Six years after the attack on Monteriggioni, in 1506, Ezio Auditore - whilst looking for a kidnapped Leonardo - learned that Saint Jerome in the Wilderness held part of a map identifying the location of the Temple of Pythagoras, and that the painting was on display in the Castel Sant'Angelo.
- Assassin's Creed II (first appearance)
- Assassin's Creed: Brotherhood