Considered to be the most beautiful place of Alexandria, it was located southwest of the city on a small hill known as the Acropolis. Its foundations were created during the reign of Ptolemy I Soter and the temple was finished during the reign of Ptolemy III.
In 48 BCE, the Medjay Bayek of Siwa visited the temple and prayed to Serapis for help on his journey. He was approached by a local priest, Lysander, who asked him to rescue a drummer who was accused by the tanner Theon of stealing a Nubian lion skin from the dye vats. The Medjay agreed to solve the situation.