Around 2296 Isu Era, Minerva, Juno, and Saturn were discussing the growing threat posed by humanity. Juno despised the humans and called for their extermination, mocking how they tried to emulate their masters. Minerva, however, argued that the species was becoming more than they were ever intended to be, even creating songs and art, and that perhaps one day they could be treated as equals. As Saturn agreed with Minerva, he was suddenly stabbed through the head by one of his human servants –  one of a number of acts which led to the outbreak of the Human-Isu War.
Filled with rage over her father's demise, Juno used the Koh-i-Noor to slaughter a man about to strike her with her father's scythe and all the revolting humans around them with tendrils of blue energy. Mad with grief, she declared that humans would destroy everything–the Isu and each other–then swore to deal with humanity by saving it from itself.
- Like his mythological counterpart, Saturn wielded a scythe. Brandished by a human after Saturn's death, the Isu weapon was shown to display some energetic power.
- In Greek mythology, Saturn's counterpart was named Kronos, the Titan father of the five or six of the twelve Olympians. He swallowed five of them to prevent a prophecy that foretold that one of his children would overthrow him. Only Zeus was spared this fate and when he grew up, he freed his siblings from Kronos' stomach. They helped him overthrow Kronos and become the new gods of the world.
- Assassin's Creed: Brotherhood (mentioned only)
- Assassin's Creed: Uprising (first appearance)
- Assassin's Creed: Odyssey – The Fate of Atlantis: Judgment of Atlantis (mentioned only)