Some time after their escape from Abstergo Industries, Lucy and Desmond Miles were joined by Rebecca and Shaun, who accompanied them as they traveled in an unmarked truck. Lucy, Rebecca, and Desmond stayed in the back of the truck, which contained a fully-functioning Animus. Though Desmond was apprehensive, Lucy convinced him to go into the genetic memories of Aquilus again. During their journey, the Assassins were attacked by Templars, who forced their vehicle from the mountain road they were on and shot their driver. Shaun, Rebecca, Desmond, and Lucy fended off the attack.
The team's doctor, Herman Geier, suggested that Abstergo had planted a bug on them, which they scoured the truck looking for, finding nothing. After Desmond had relived more of Aquilus' memories, he made everyone stop and get out of the van, where he asked for Shaun's gun and shot Geier in the leg. They used a phone taken from the body of one of their Templar attackers and dialed the last known contact, causing Geier's phone to ring. Desmond killed Geier with a bullet to the head, and they continued on their way to Monteriggioni.
In the Villa Auditore, Rebecca told Lucy to not neglect her feelings for Desmond, but Lucy shut down the conversation. After Desmond discovered the location of the Ankh, the Assassins discussed what their next step would be, when they were interrupted by a call from Stella Crow and the arrival of Jonathan Hawk.