The Pythagorean Vault was a small Temple constructed by the Isu that was later incorporated into the Temple of Pythagoras, in Rome. Small in comparison to many other Temples, the Pythagorean Vault was largely hollow, save for a small pedestal located in the center of the main chamber on a raised platform.
In 1506, Ercole Massimo and his Hermeticists sought to enter the temple and the vault. To do this, they kidnapped Leonardo da Vinci and tortured him to help them gain entrance. However, Ezio Auditore da Firenze, the Mentor of the Italian Brotherhood of Assassins, rescued Leonardo and slew Ercole. Afterwards, Leonardo and Ezio explored the Pythagorean temple and entered the vault. Once they activated the pedestal within the vault, the walls of the vault lit up with numerous symbols, numbers, and letter codes: "43 39 19 N, 75 27 42 W". Though Leonardo thought that the sequence was meaningless, Ezio simply remarked that it was not meant for them.
- The numbers revealed in the Pythagorean vault are the latitude and longitude of Turin, New York, where the Grand Temple is located.
- Assassin's Creed: Brotherhood - The Da Vinci Disappearance (first appearance)
- Assassin's Creed: Project Legacy