In legends, Osiris and his wife Isis watched over the Egyptian pharaohs, with Isis using a Piece of Eden to keep them from harm. However, Osiris died after some time, murdered by his brother Seth, causing Isis to be overtaken by grief and despair.
During the 1st century BCE, whenever the Medjay and later Hidden One Bayek of Siwa slew a member of the Order of the Ancients, he told them that Osiris awaited them to judge them for their crimes against humanity.
- Historians during the classical antiquity viewed Osiris as the Egyptian counterpart to Aita, Hades, and Pluto as they were all major chthonic deities. Serapis was a syncretism formed by these cultures.