During the 1st century BCE, Nitokris served as the latest God's Wife of Amun in the temple of Karnak in Thebes. During her tenure, tombs in the area were often desecrated by tomb robbers, who robbed its riches. Determined to end the desecration, Nitokris sought to conduct an ancient Ma'at ritual to deter the tomb robbers, visiting the Necropolis of the Nobles in the Theban Necropolis for more information. However, her attempt was discovered by a group of tomb robbers, who had her murdered.
After her death, Nitokris was buried in the very same tomb. The Apple of Eden under her care was also passed down to Isidora, who went on to pursue vengeance against the tomb robbers by unleashing a "curse" onto the city.
- Assassin's Creed: Desert Oath (first appearance)
- Assassin's Creed: Origins – The Curse of the Pharaohs (mentioned only)