Mitsuko Nakamura is the Director of Research of Abstergo Industries' Lineage Discovery and Acquisition, a division based in Philadelphia. As such, she is also a member of the Inner Sanctum, which acts as both an elite council for the Templar Order and the board of directors for Abstergo Industries.
On 16 December 2013, Nakamura was notified of the discovery of Milton Jones' ancestral ties to the Assassin Adéwalé by Dr. Damian Saravakos, revealing that while they may not find new artifacts in his memories, the genetic memory would be valuable to the employees at Abstergo Entertainment.
On 15 October 2015, Nakamura had a conference with Isabelle Ardant, and a year later was present during the initiation of Ardant's successor, Simon Hathaway, into the Inner Sanctum. Ten days later, Nakamura was among those who voted to clear Hathaway of all the charges brought against him, convinced by his speech and that his shady actions were in fact carried out for the benefit of the Templar Order.
In March 2017, the Inner Sanctum invited the new Black Cross to a meeting in Quebec, during which Nakamura was present. Displeased that he auto-proclaimed himself their inquisitor, they threatened him and demanded to know his identity. The masked man responded that he came only as a courtesy, to inform them that he was investigating a hostile fifth column within the Order. After affirming his independence, the Black Cross dropped a smoke bomb and disappeared. 
In November, the Templar fugitives Thomas Adler and his wife Alice tried to hack Nakamura's Abstergo account to learn more about Project Rainbow, as the access to the intel about the 1943 secret project was reserved to Inner Sanctum members only. However, the Adlers failed to do so and they instead hacked the account of Alan Rikkin, who recently died and whose account was consequently less protected.
- Despite Mitsuko being a feminine given name of Japanese origin, in the non-canonical modern day storyline of the French Assassin's Creed comic series, Nakamura is depicted as a man and is responsible for giving the direct orders to the Templar agent Vernon Hest. Assassin's Creed: Heresy would later confirm that Nakamura is indeed a woman canonically.
- Assassin's Creed: Revelations (mentioned only)
- Assassin's Creed: Initiates (mentioned only)
- Assassin's Creed: Syndicate (mentioned in Database entry only)
- Assassin's Creed: Heresy
- Assassin's Creed: Uprising
- Assassin's Creed: Conspirations
- Assassin's Creed (Les Deux Royaumes comics) (first appearance)