The Libyan Brotherhood of Assassins were the branch of Assassins located in Libya.
Sometime before 1805, the Assassin Ahkbar was sent to spy on Ottoman Sultan Selim III, who was in possession of a Piece of Eden called the Koh-i-Noor. Eventually, Ahkbar managed to gain the sultan's trust and became one of his spies. At some point, Tavis Olier, the Black Cross, infiltrated the Sultan's palace in Tripoli in an attempt to retrieve the Koh-i-Noor. However, Tavis was caught and, despite Ahkbar's suggestion to kill him, Selim decided to imprison Tavis in case he would ever need him as a bargaining chip.
In 1805, Ahkbar tricked Jan van der Graff, an agent of Napoleon, and Solomon Bolden, the new Black Cross, by giving them directions towards a tunnel where he and several men ambushed them, which resulted in Ahkbar killing Bolden and van der Graff being captured. Afterwards, Ahkbar supervised van der Graff's interrogation, eventually concluding that, unlike Bolden, he was not a Templar and knew nothing of the true power of the Koh-i-Noor. Doubtful, the Sultan decided to keep van der Graff alive and had him thrown into Olier's cell, who then trained the younger man in the Templar ways.
In 1808, the Assassins informed Selim that they intended to buy the Koh-i-Noor and expected him to deliver the relic to them. However, Selim refused to hand over the relic, believing that its power would allow him to withstand both the Assassins and the Templars. However, on 28 July 1808, Ahkbar poisoned Selim and took possession of the Koh-i-Noor. Shortly after, van der Graff, who had managed to escape thanks to Olier's sacrifice, arrived in the room. During their conversation, Ahkbar revealed his intention to betray the Brotherhood and keep the relic for himself. Ahkbar tried to use the Koh-i-Noor and its power to create illusions, but Graff saw through it and killed him.
Afterwards, the Assassins arrived and revealed that they had overheard van der Graff's conversation with Ahkbar. After declaring that Ahkbar had no honor and that his death was not an affront to the Brotherhood, the Assassins asked van der Graff what he intended to do with the relic. To their surprise, van der Graff handed them the box containing the artifact, stating that he did not affiliate himself with the Templars and had only killed Ahkbar to avenge his friends. After declaring that he was now done with it, van der Graff told the Assassins to take the box and leave, which they did.
However, the box given to the Assassins was empty, as van der Graff had taken the Koh-i-Noor with him, having lied about his loyalties.