In April 1865, with the Confederate Army losing the American Civil War, Booth planned the murder of President Lincoln along with several other political figures. However, only Booth was successful, shooting Lincoln in the back of the head before fleeing the scene. He proclaimed "Sic semper tyrannis!", thus always to tyrants, copying Marcus Junius Brutus' words after Caesar's assassination.
Twelve days later, Booth was cornered by federal troops in northern Virginia, but refused to surrender. The soldiers set fire to the barn, and in the confusion, Booth was killed by the Assassins.
Details of Booth's assassination were later collected by the Assassin Clay Kaczmarek in 2012, and hidden in the Animus for his successor, Desmond Miles to find. Which he did in September of that year. Desmond solved this puzzle, which was part of a set titled "Guardians" where Booth was included in the list of historical individuals assassinated.
Behind the scenes
John Wilkes Booth is a historical figure and figure introduced in Assassin's Creed II in the Glyph puzzles. Historically, Booth once portrayed the Roman general Marcus Antonius in a production of Shakespeare's Julius Caesar; additionally, his father and brother, both fellow actors, were both named Junius Brutus, after Caesar's assassin.