Serving the Borgia
Some time after Fiora and Baltasar had tailed and studied the Assassins, they both attempted to "create" their own. With this in mind, the two trained Il Lupo to master everything they had witnessed about their enemies, such as their movement and assassination techniques.
- "With Baltasar dead, Il Lupo remains the only one who can tie me to any harm done to the Assassins."
- ―Fiora, just before seeking out Il Lupo.[src]
When she was ready, Fiora left a signal on the Pasquino statue as a message to Il Lupo that she wished to meet him. Following this, Fiora waited for him at the docks at sunset, with an Assassin tailing her for her protection, as she had only recently escaped an attempt on her life by Baltasar. However, Il Lupo quickly killed the Assassin and threw their body into the river, before telling Fiora calmly that she had been followed.
Making up a bluff, Fiora began to tell him of an "assignment" from Cesare, which was to eliminate a target on a boat out in the harbor. As Il Lupo turned away from her to look towards the boat, and questioned how he would be able to reach it unseen, Fiora ambushed him.
With a surprise attack, she cut into his back with a poisoned, iron-tipped fan, though Il Lupo retaliated, backhanding her and drawing his rapier. However, his efforts were in vain, as he quickly succumbed to the poison; vomiting, cursing and questioning Fiora as he died.
- The grey of Il Lupo's cloak evoked a wolf's coat, and the "fang-like" blades resembled a wolf's fangs, concealed right before the strike.
- In the Assassin's Creed: Brotherhood official strategy guide (Collector's Edition), Il Lupo was shown with an actual Hidden Blade, instead of his switchblade, though the Hidden Blade was worn on his right arm.
- Il Lupo's name structure was similar to La Volpe's. In addition, both of their clothing and personalities resembled their animal namesakes.