Saint-Domingue had been a part of France's colonial territories in the New World since 1625, where she shared the island of Hispaniola with the Spanish colony of Santo Domingo. By the middle of the 18th century, the Assassin Brotherhood had established a permanent presence in the colony. Under the leadership of their Mentor François Mackandal, a revolutionary Houngan, the Assassins consisted primarily of African slaves opposing the white colonists of Saint Domingue.
In 1758, François Mackandal was captured and executed after a failed poisoning attempt on the white colonists of Saint-Domingue. Mackandal's students Agaté and Baptiste both individually fled Saint-Domingue and ended up in the French colony of Louisiana.
In 1957, Templar-affiliate François Duvalier was elected President of Haiti, and became responsible for more than ten years of corrupt and murderous leadership. On 21 April 1971, Duvalier was killed by the Assassins.
- The names of the French colony Saint-Domingue, and the Spanish colony of Santo Domingo, which between themselves divided the island of Hispaniola, both translate to "Saint Dominic", a Spanish friar of the Dominican Order.
- Assassin's Creed II (mentioned only)
- Assassin's Creed III: Liberation (mentioned only)
- Assassin's Creed IV: Black Flag – Freedom Cry (first appearance)