During the Age of Discovery, several European explorers tried to find and claim the fountain as their own. In 1521, the Templar-influenced Spanish explorer Juan Ponce de León journeyed to Florida to obtain the artifact. The Assassins in Florida were determined to stop Ponce de León, and sent Miguel Ramón Carlo de Lugo to convince him to abandon his quest and turn back. When Ponce de León refused, the Assassins struck him with a poisoned arrow that would later result in his death.
- Assassin's Creed: Revelations – Discover Your Legacy (first appearance)
- Assassin's Creed IV: Black Flag (mentioned only)
- Assassin's Creed: Black Flag (mentioned only)
- Assassin's Creed IV Black Flag: Blackbeard – The Lost Journal (mentioned only)