Eden, or the Garden of Eden, was an ancient city built by the Isu. There, they held many of their creations, humanity, whom they used as a docile workforce and enslaved through technology later known as the Pieces of Eden.
Adam and Eve were believed to have been the first humans capable of resisting the power of the Pieces, presumably because they were devoid of the neurotransmitters that the Isu had implanted into their creations. The two were known to have tried to escape Eden through freerunning, after having stolen one of the Apples of Eden, although they were stopped by an unknown force.
In modern times, Clay Kaczmarek, a descendant of Adam, revealed Adam and Eve's escape to his successor in the Animus Project, Desmond Miles, through a video file named the Truth; this video showed glimpses of Eden.
Later on, after Desmond had recovered all of the glyphs and solved the rifts in the Animus' programming, he encountered an executable file dubbed "the Miracle" by Clay. Through loading it in the Animus Virtual Training Program and discovering the path to navigate, Desmond encountered Clay's virtual copy, who told him "Eden. She... in Eden. Find Eve. The key. Her DNA...", referencing the location.
- The garden of Eden (Hebrew גַּן עֵדֶן, Gan ʿEḏen / Arabic جنات عدن, Jannat Eadn) appears in the Bible, Talmud and Quran as the primeval garden where God settled the first two humans: Adam and Eve. Eden is thought to derive from an Aramaic root meaning 'fruitful, well-watered'.
- The mountain behind Eden strongly resembles Mount Kilimanjaro, found in eastern Africa.
- Eden greatly resembles the common idea of a futuristic 'green city', with roofs used to grow greenery, implying the environment was important to the Isu.