- "The Daughters of Artemis are strong here. I'll be on my guard."
- ―Kassandra in Malis[src]
The Daughters of Artemis favored hunting camps set deep in the woods or near places sacred to Artemis. Occasionally, these camps became larger settlements, most notable among them being their original home of Huntress Village on the island of Chios, and the village of Lamia in Malis.
The emblem of the Daughters of Artemis was a stylized figure of a woman drawing a bow, presumably a depiction of Artemis. They wore furs and animal skins, and had often painted red markings on their skin.
The Daughters seemed to favor staying out of the Peloponnesian War and stuck to their settlements and the wilderness. However, they were known for harassing the common folk, usually by releasing the latter's animals, but also by threatening those who hunted in 'their' forests. They also sometimes disturbed religious practices or downright destroyed statues and shrines.
In 431 BCE Daphnae, the leader of the Daughters at the time, tasked the Spartan misthios Kassandra to hunt several legendary creatures found across Greece. Daphnae later revealed that this process was to test a new candidate to lead the Daughters of Artemis; it would culminate in the challenger and the ruling leader fighting to the death. The winner would be the leader of the Daughters, until another challenger arose.