Daphne was born in Cyprus, living later in both Paris, France and London, England. She studied Law at Somerville College, Oxford before training to be an actress at LAMDA. After graduating she rose to prominence playing Nurse Talianos on the BBC medical drama Casualty in 2007.
Assassin's Creed: Odyssey
Daphne Alexander voices the Greek hetaera Anthousa and several civilians in Assassin's Creed: Odyssey, released 5 October 2018. Daphne was selected by Ubisoft because of her Greek heritage and Ubisoft's desire to cast ethnic Greeks and native speakers.