Around the time of the Peloponnesian War, the Daughters of Artemis learned that legendary beasts had been seen throughout the Greek world again. Taking this as a sign that her position as a leader of the Daughters was challenged, Daphne came to the dilapidated Temple of Artemis in Phokis. While there, she met the Spartan misthios Kassandra, who proved her worth by killing the Kalydonian Boar nearby. Awestruck, Daphnae tasked Kassandra to hunt the rest of the legendary creatures, telling her to bring their pelts to her as proof.
After the last beast had fallen, Daphnae moved to the island of Chios, where a village of the Daughters was located. Leading the misthios to meet a group of gathered Daughters, Daphnae revealed that by successfully slaying the beasts, Kassandra had established herself as a challenger for the position of the leader of the Daughters. The challenger and the acting leader were then to fight to the death in order to decide the leader of the Daughters of Artemis.
Behind the scenes
In Assassin's Creed: Odyssey, if Daphnae is slain, the Eagle Bearer becomes the new leader of the Daughters of Artemis, and as such, free to wander through their campsites without drawing their ire. If Daphnae is spared, the romantic relationship the Eagle Bearer may have entered with her will be broken, the Eagle Bearer becoming an anathema to the Daughters and banished from Daphnae's sight.