- "They had these tools, these powerful machines, that could predict possible futures. Not what was going to happen, but wha-what could happen... probabilities. And... well, they spent a lot of energy trying to decide what was the most likely scenario for the future. Theirs and ours."
- ―Desmond Miles attempting to explain First Civilzation calculations.[src]
Minerva's calculations made the Isu proficient in the studies of alternate, future, timelines. From within the Nexus, Jupiter, Juno and Minerva worked to influence several individuals amongst humanity in the future to prevent the recurrence of a solar flare from devastating the planet after seeing one coming in their own near future.
- Minerva constructed two builds of the Eye based on her studies; the first, to alter existence itself and prevent the Second Disaster, and the second to commune with Desmond Miles, through his ancestor Ezio Auditore da Firenze.
- Ezio Auditore da Firenze once used his Apple to discern what threat Cesare Borgia still posed following his arrest. What he saw immediately resolved him to eliminate the incarcerated Grand Master.
- Ratonhnhaké:ton communed with Juno via a Crystal Ball; she instructed him to join the Assassins, and showed him the consequences should he not do so: the Templars would breach the sanctuary near Kanatahséton, and the world would not be saved from the coming apocalypse.
- Later, an Apple of Eden also showed Ratonhnhaké:ton that if he had never become an Assassin, he would not have been present to prevent George Washington's corruption, and the collapse of the United States into civil war.
- When Nikolai Orelov touched the Imperial Sceptre – a Staff of Eden – during the Tunguska event, he saw events from the past and future. Through the Bleeding Effect, his descendant Daniel Cross saw his eventual meeting with the Mentor.
- Inside the Colosseum Vault, Juno showed Desmond Miles what would happen if he did not kill Lucy Stillman: Abstergo Industries would have arrived to claim Ezio's Apple of Eden and placed it in the Eye-Abstergo satellite, dismissing its importance in preventing the Second Disaster.
- When Clay Kaczmarek merged with Desmond Miles, the latter learned of the former's knowledge of the calculations, which he later documented in a cell phone message memo. In the message, he noted several versions of himself: one who got married early and had a son, the reality who stayed single in New York, one who went to San Francisco to be a waiter, one who got a job at an auto body shop in Chicago, and one who never left The Farm in the first place.
- Minerva later showed Desmond that if he did not use the Eye and release Juno from the Grand Temple, then the cataclysm would have destroyed humanity, but that he would lead the survivors in a restart of civilization. Long after his death, history would have repeated itself; Desmond would be worshipped as a god, but his teachings would be twisted to justify mass genocide.
- In the 5th century BCE the Isu Aletheia explained to Kassandra (and through her, Layla Hassan) that the Isu had spent so much time looking for alternate futures where "everything remained the same", rather than ones where the Isu and humans might work together to survive, that it ultimately cost them any chance of survival.
- Darby McDevitt has stated that "Calculations" is the in-house term for parallel universes in the series, and added that Clay Kaczmarek went insane because he saw too many possible futures, in addition to the Bleeding Effect.
- Assassin's Creed: Brotherhood
- Assassin's Creed: Revelations (First mentioned)
- Assassin's Creed III (mentioned only)
- The Tyranny of King Washington (first appearance)
- Assassin's Creed IV: Black Flag
- Assassin's Creed: Odyssey (mentioned only)