The manga will center on Shao Jun, the protagonist of the Assassin's Creed Chronicles: China game, and the last Assassin in China. Set in Ming dynasty China, the emperor enacts political purges that begin to blow the winds of unrest. After her comrades are all killed, Shao Jun flees to Europe to train, and then returns to China to exact her revenge.
Announced in September 2019 via the Sunday-GX Twitter account with art by Minoji Kurata based on story treatment by Ubisoft, the manga debuted in the magazine's November issue on October 19. The first tankōbon volume was released in Japanese on 19 February 2020 by Shogakukan. The cover and two preview pages of the first volume were released on 16 February 2020. English and French language translations were confirmed to come later, with the French version published by Mana Books released on 11 June 2020,, the German version published by Tokyopop released on 1 September 2020 and the English version by Viz Media released on 16 February 2021. On 6 June, Mana Books released a trailer and preview of the first chapter online. On June 9, Mana Books released a documentary dedicated to Minoji Kurara on the production of the book. 
The release of Chapter 16, originally scheduled to release in January 2021, was delayed a month, Kurata cited the underestimated workload as the reason. On 19 May, Kurata confirmed that Blade of Shao Jun would end serialization the following month, making the total number of chapters in the series 20.