The Appennine Mountains was a mountain range that stretched from north to south, along Italy's east coast. The mountains linked Florence to both Romagna and Forlì. Several hamlets, farms, and a church could be found in the mountain range.
In 1480, Leonardo da Vinci was offered a commission in Venice, and thus took a carriage into the Appennine Mountains during his journey from Florence to the port in Romagna. After assassinating Jacopo de' Pazzi, the Assassin Ezio Auditore da Firenze followed the artist into the range.
There, Ezio found Leonardo alongside his broken cart, where he was attempting to fix the shattered wheel. The Assassin helped him repair the carriage, and caught his first glimpse of Leonardo's Flying Machine, which was loaded into the back of the cart.
Together they set off for Forlì, from where they would take a boat to Venice. However, Borgia guards on horseback pursued them through the range, setting fires in front of them, and attempting to board the carriage itself.
The two managed to evade their attempts, and upon reaching the mountain range's end, Ezio told Leonardo to go ahead in the carriage, while he stayed to take care of the guards.
- The Appennine Mountains was the only location to be entirely devoid of guards, shops, and civilians.
- The Appennine Mountains was the only location in Assassin's Creed II to feature a piece from the Assassin's Creed soundtrack, entitled "Dunes of Death."
- The Appennine Mountains contained 16 treasure chests.