Alfonso's second wife was Lucrezia Borgia, daughter of Pope Alexander VI, Grand Master of the Italian Templars. After Alexander's death, the secret Assassin Lodovico Ariosto lived with the Este family as the Ferraran ambassador of the Vatican.
- Assassin's Creed: Project Legacy (mnemonic set only)
- Assassin's Creed: Brotherhood – The Da Vinci Disappearance (mentioned only)
- Assassin's Creed: Revelations novel (mentioned only)