During the Renaissance, the Assassin Ezio Auditore da Firenze bought a replica of Dürer's painting Eve. In 1505, Dürer was offered an enormous sum of money to paint a portrait of Sofia Sartor as a gift from her father.
On seeing her however, he refused to receive any payment, claiming that the honor of painting her was payment enough. After Sofia had sat for the portrait for a week, Dürer finished it. The portrait had notable warmth and color, and was titled Portrait of a Venetian Woman. In 1511, this portrait was stolen from Sofia's bookstore in Constantinople and sold to a merchant. Ezio, an acquaintance of her, would later steal it back.
In 2012, Dürer's woodcut engraving Cain Killing Abel was included in the Glyph puzzles the late Assassin Clay Kaczmarek had left behind in the Animus as messages for his successor Desmond Miles to decipher. Desmond later did in September of that year. Desmond solved this puzzle, which was part of a set titled "Brothers" learning that Cain killed his brother to attain his Apple of Eden.
Behind the scenes
Art Director for Assassin's Creed: Revelations, Raphael Lacoste, wanted to base Sofia's appearance on Dürer's portrait, and co-artist Jeff Simpson commented that Lacoste was very adamant about the idea.