Der Assassin's Creed: Brotherhood Soundtrack wurde von Jesper Kyd komponiert und enthält 20 Titel im Original Game Soundtrack und 22 in den Spezial Editionen.
Titel (Original Game Soundtrack)[]
- Master Assassin (3:21)
- City of Rome (5:30)
- Cesare Borgia (2:21)
- Flags of Rome (2:36)
- The Brotherhood Escapes (1:58)
- Brotherhood of Assassins (3:03)
- The Pantheon (3:06)
- Villa Under Attack (2:13)
- Echoes of the Roman Ruins VEREINT EUCH (2:51)
- Borgia Tower (2:14)
- Borgia Occupation (3:02)
- Roman Underworld (3:46)
- Countdown (3:30)
- Borgia - The Rulers of Rome (3:57)
- Ezio Confronts Lucrezia (2:56)
- Battle in Spain (1:39)
- Fight of the Assassins (2:38)
- Desmond Miles (4:41)
- VR Room (2:43)
- Apple Chamber (4:55)
Titel (Collector's und Limited Kodex Edition Soundtrack)[]
- Borgia Occupation (3:03)
- Master Assassin (3:21)
- Cesare Borgia (3:01)
- Infiltrating the Borgia Castle (2:36)
- City of Rome (5:33)
- The Brotherhood Escapes (2:03)
- Brotherhood of Assassins (3:04)
- The Pantheon (3:06)
- Villa Under Attack (2:13)
- Echoes of the Roman Ruins (2:52)
- Rome Countryside (3:01)
- Borgia Tower (2:13)
- Roman Underworld (3:45)
- Countdown (3:35)
- Borgia - The Rulers of Rome (4:00)
- Ezio Confronts Lucrezia (2:59)
- Legacy of the Borgia Family (2:31)
- Battle in Spain (1:36)
- Fight of the Assassins (2:38)
- Desmond Miles (4:47)
- VR Room (2:49)
- End Fight [Assassin's Creed II Bonus Track] (1:38)