Juno

Juno, previously known as Uni, was a member of the First Civilization. She is portrayed as a holographic form, appearing four times to Desmond Miles throughout the end of Assassin's Creed: Brotherhood after completing the path through the Colosseum. First after passing through the first hidden entrance, second while Desmond is approaching the second hidden entrance, third after entering and flipping the switch inside the Santa Maria Aracoeli, and lastly inside the Vault beneath the Santa Maria Aracoeli. Juno further expands Minerva's warning at the end of Assassin's Creed II, though her dialogue is also noticeably more hostile in tone towards humanity than Minerva's. Once Desmond, Lucy, Shaun and Rebecca reach the Apple's location in the Vault, Juno forcefully takes control of Desmond, causing him to unwillingly strike Lucy with his Hidden Blade.

Dialogue
After passing through the first hidden entrance

Juno: ''We commit this space to the epilogue of our ending. Let it be found by he who is deemed worthy. Let it guide him. Let it shape his path forward. Let it save the world we leave behind.''

Desmond: Who are you?

After approaching the second hidden entrance

Juno: ''In the beginning, we set our truths to parchment. To stone. To the memory of men. These proved impermanent things. Cleansed by fire. Cleansed by famine. Cleansed by flood. All the world is innocent once more. Innocent and ignorant.''

After flipping the switch inside the Santa Maria Aracoeli

Juno: ''We did not build them to be wise. And now they are our final, faulted hope. You are they. You possess the potential for understanding. But you broke our tools. Or turned them against one another. We have destroyed what we could. Sealed away what we could not. Most. Not all. And it does not take many to unwind the world. Here is a safe place. Eternal. To store objects. Words. Wisdom. But not life. Almost did we have the means. But time... time erodes us. We can distract him. We can see past him. Feint left when he strikes right. But his reach is so very long. His stamina unending. We cannot evade his grasp. Not forever.''

After entering the vault beneath the Santa Maria Aracoeli

Juno: ''A hundred years I might speak and still you would not know us. You with five senses. Us with six. The one we kept from you. To be safe. Now, you can never know. Only try. Grasp. You can SEE. SMELL. TASTE. TOUCH. HEAR. Knowledge has been blocked away. After, when the world became undone, we tried to pass it through the blood. Tried to join you with us. You see the blue shimmer. You hear the words. But you do not know. WE SHOULD HAVE LEFT YOU AS YOU WERE. It is hard to stay contained. Knowing as we do. We wait for you, Desmond. You will come here. You will activate it. You will know only when it is too late.''

After activating the Apple

Desmond: ''What's happening? I cant move!''

Juno: ''Your DNA communes with the Apple. You have activated it.''

Desmond: Let me go!

Juno: ''On the 72nd day before the moment of awakening. You, birthed from our loins and the loins of our enemies. The end and the beginning, who we abhor and honor. The final journey commences. There is one who would accompany you through the gate. She lies not within our sight. The cross darkens the horizon.''

Desmond: What are you doing?

Juno: ''The Path must be opened. You cannot escape your part in this. The scales shall be balanced.''

Desmond: ''Stop. Please...''

Juno: ''You know very little. We must guide you. Cease your struggle.''

Desmond: No! (Desmond stabs Lucy in the stomach)

Juno: ''It is done. The way lies all before you. Only she remains to be found. Awaken the sixth. Go. ALONE!''

Trivia

 * In Roman mythology, Juno's epithet Moneta is translated as "the one who warns", which may be related to the sound warnings she gives to Desmond about his future and "the Cross" which likely refers to Abstergo.
 * Lucina, another of Juno's epithet, means "she who brings children into light", which also may be related to her reference to her people's sixth sense.
 * She has also been referred to as Pomona, meaning goddess of fruit, which may hint at her possession of an Apple of Eden sometime in her life.
 * Juno was the patron goddess of Rome and the Roman empire.
 * Juno is equivalent to Hera, one of the Greek goddess.
 * Juno's in game model shows her wearing a belt that is shaped similarly to the Assassin symbol; this may be the inspiration for the early Assassins adopting this symbol as their own.